Bad Or Good Essay Research Paper Bad free essay sample

Bad Or Good Essay, Research Paper Bad or Good We live in a universe in which our primary nutrient is the information. We perceive the outside universe through images, and each image has an reverberation in our encephalon, bring forthing feelings, attitudes and sometimes inquiries. Although we belong to the same coinage, 1000s of differences or similarities divide and classify us. Each of us perceives in his ain manner the information he receives. For some of us, something could be beautiful, for others the same thing could be ugly. Behind these two words, beautiful and ugly, we can see another words or, better said, constructs: good and bad. But what do good and bad mean? Do they truly be? On one manus, good and bad are two words that express our sentiment in footings of perceived images. We give the images values, which can be, as I already said, good or bad. On the other manus, being so many types of human existences, it is normal to be different sorts of perceptual experience and reading of information. Therefore, holding many people who can construe things i n different ways, it is hard for person to state what is good or bad, and in the same clip to hold his sentiment shared by everyone. Trying to sort, we might come up with a consequence that might be true or non, depending on the point of position. In other words everything is comparative. Paradoxically, when we start believing we, we discover that we really cognize about nil, or that there are many things left, to be known. On the other manus, the more we know, the more we want to cognize. This desire of cognizing more and more might be expressed through inquiries. One large difference that separates the human existences from animate beings is that people ask themselves, why do we be? and animate beings do non. If animate beings asked themselves this inquiry, in that minute they would go human existences. If person find an reply to this inquiry, many things would be simplified. Our secret of life would non be a secret anymore. However, replying this inquiry is hard, possibly impossible. What we can make is to wait and believe. Time will reply us.

Free Essays on Absent Fathers

Absent Fathers: How it affects the kids? Absent fathers is the most abused social problem. When a kid’s father dies, there is no solution unless the mother gets remarried. When the father is alive and knows he is your father, but neglects to care for or acknowledge his kid, is what is being abused. No good comes from a life without a father. Two parents are better than one without question. If one were to believe that any good can come when you raise children without a father, my question is, why? How do you? How do you expect a kid to go out in a world from a fatherless family, and adapt into a rather masculine world? How do you believe that being raised with no father doesn’t damage a kid’s perspective of life? Do you think that a kid will get the same amount of attention, love, emotion, or interaction that he/she would in a two parent household? Do you believe that children can develop everything they need with only a mom, when another child can gain so much more from both parent figures that just a mother? Mothers and fathers obviously parents differently. If children have the combination of what mothers and father bring to parenting, they will have a fuller perspective on life and how to deal with certain situations. Mothers are affectionate, tend to be more comforting, emotional, and enforce the safety of their children. Fathers concentrate alot on discipline, raising obedient children, enforcing success, and a father figure is more physical. When little boys see how fathers treat their mothers or how their father deals with certain situations, do you think it affects them in the way they will treat people, or deal with situations in the future? When little girls see the love, and attention, and protection their father provides, they will know what to expect in a future relationship with another man (Horn). With one parent in a home the stress is overwhelming. How do they have time to fit everything in, from... Free Essays on Absent Fathers Free Essays on Absent Fathers Absent Fathers: How it affects the kids? Absent fathers is the most abused social problem. When a kid’s father dies, there is no solution unless the mother gets remarried. When the father is alive and knows he is your father, but neglects to care for or acknowledge his kid, is what is being abused. No good comes from a life without a father. Two parents are better than one without question. If one were to believe that any good can come when you raise children without a father, my question is, why? How do you? How do you expect a kid to go out in a world from a fatherless family, and adapt into a rather masculine world? How do you believe that being raised with no father doesn’t damage a kid’s perspective of life? Do you think that a kid will get the same amount of attention, love, emotion, or interaction that he/she would in a two parent household? Do you believe that children can develop everything they need with only a mom, when another child can gain so much more from both parent figures that just a mother? Mothers and fathers obviously parents differently. If children have the combination of what mothers and father bring to parenting, they will have a fuller perspective on life and how to deal with certain situations. Mothers are affectionate, tend to be more comforting, emotional, and enforce the safety of their children. Fathers concentrate alot on discipline, raising obedient children, enforcing success, and a father figure is more physical. When little boys see how fathers treat their mothers or how their father deals with certain situations, do you think it affects them in the way they will treat people, or deal with situations in the future? When little girls see the love, and attention, and protection their father provides, they will know what to expect in a future relationship with another man (Horn). With one parent in a home the stress is overwhelming. How do they have time to fit everything in, from...

A Framework Model for an Online Examination Timetable using Constraint Dissertation

A Framework Model for an Online Examination Timetable using Constraint Programming, PHP and MySQL - Dissertation Example The challenge and complexity of the problem lies in the fact that institutions may need to satisfy a set of constraints that might be too diverse or even contradictory. There are a few constraints that cannot be violated at all (hard constraints), few constraints are non universal (soft constraints) and may or may not be followed by an institute and lastly, there may be constraints unique to a specific institute (Burke et al. 1995). Problem Statement Academic institutions all over the world are required to go through the tedious and time consuming task of producing examination timetables periodically. Therefore, a universal solution for the examination timetabling problem would have a substantial impact factor. Owing to the fact that different institutes require a solution satisfying different constraints, the problem of finding a generalized solution that caters all these differences could be rather challenging. Devising a universal model for examination timetable problem would requ ire flexibility in terms of the specified constraints and commercial software cannot provide that. Aims and Objectives The aim of this project would be to suggest a universal framework model for the examination timetabling problem. A solution that ensures provision of flexibility in terms of constraint specifications shall be proposed. ... Literature includes timetabling systems presented by Hansen and Vidal (1995), Colijn and Layfield (1995), Lim et al (2000) and Dimopoulou and Miliotis (2001). Various approaches have been suggested by researchers and universities to solve the examination timetabling problem. Some survey papers have been published over time listing the techniques that have been utilized in addressing the exam timetabling problems. These include the survey by Carter and Laporte (1996), Burke and Petrovic (2002), Schaerf (1999), Petrovic and Burke (2004) and Burke et al. (1997). Amongst the approaches include methods based on evolutionary algorithms (Cote 2005), clustering, graph based sequential methods, case based reasoning (Gaspero & Schaerf 2001), hyper heuristics (Burke et al. 2007), harmony search algorithms (Burke et. al 2004), tabu search (Gendreau & Potvin 2005), particle swarm algorithms (Gaspero & Shuref 2001), and simulated annealing (Chiarandini 2006) have been proposed for the examination timetabling problem. It has been observed that hybrid methods in general give better solutions that pure algorithms. However, efficient integration is required rather than sequentially integrating the different approaches (Que et al. 2006). For building timetabling systems, researchers have used some general constraint programming packages e.g. ECLiPse (Ajili & Wallace 2003). A few efforts have been seen in literature for standardizing the modelling language and data format (Kingston 2001; Ozcan 2003; Reis & Oliveira 2001) once the need for it was recognized (Burke et al. 1998). Methodology The project has both research and development phases, so time shall be divided accordingly. The framework model would have the server-client architecture and would comprise of

Current Issues in PR Essay Example | Topics and Well Written Essays - 6500 words

Current Issues in PR - Essay Example The issue of water has emerged as a global, ethical and environmental issue which is primarily driven by economic. The modern age consumer is questioning the international connections, pollution and water usage. The environment and the economics of the operation is challenged by the consumer. The desire to make informed choice is inherent in any consumer choice, and hence one of the current issues in PR has been the awareness in relation to the ‘Bottled Water’ as in the recent years the consumption of bottled water has increased 200 times, which is remarkably substantial. the implications of countries effectively exporting their water in the forms of food, computers, clothing and cars. For example, Britons use on average about 150 litres per day. If you include embedded water that rises to 3400 litres a day. This illustrates the obvious need to look at the use of water right across the supply chain. Since agriculture uses most of the world’s fresh water resources, perhaps we should be calculating the â€Å"water footprint† of food as well as its â€Å"carbon footprint†.† http://www.developpement-durable.veolia.com/library/fr/standalone/publications/rapports-environnementaux/1802,Rapport-Resp.-Sociale-Veolia-Eau-UK.pdf For the Water Working Group at the World Business Council for Sustainable Development (WBCSD) to develop a mapping tool to help businesses assess their water footprints and use this data to assess the risks in relationship to the current and future availability of water. They have also indicated a need to develop also global governance platform to deal with changing water scenarios. There has been predictions, which lead to belief that in future water scarcity, may emerge as on of the most potent cause of conflict and war. A sound PR campaign needs to be developed, as so many people are impacted by it and it has grown substantially from 1970 raising its

Ethical Decision Making Case Study Example | Topics and Well Written Essays - 1000 words

Ethical Decision Making - Case Study Example Before deciding which way to advice Elsie, Jones will, first of all, have to consider all the facts surrounding the matter. Being the Christian believers they are, abortion is not only a crime but also, and more importantly, a sin(Boyle 1). The act of procuring the abortion amounts to murder. If Elsie procured it, their relationship with God would not be right. Given that Elsies Uncle is a strict disciplinarian, it is uncertain how they would react to the news of Elsie’s pregnancy, let alone procuring an abortion. On health grounds, procuring an abortion could damage Elsie’s uterus so that in the future, they may never again conceive. Secondly, Jones will have to consider all the possible courses of action available to Elsie. First, they could give in to the pressure of the boyfriend and go for abortion. Secondly, they could carry the pregnancy to its full term. Then, upon giving birth, if they did not wish to raise the child, they could give them away for adoption(Boyle 3). However, like the first option, the second option too has consequences. For instance, it is not known how Elsie’s Uncle would react. Besides, the pursuit of this option is likely to disrupt Elsie's studies as they will be forced to take maternity leave. Today society may have reached the point where it is normal for a woman to procure an abortion. However, Elsie, Jones and their families, besides being members of this society, are Christian believers. Christian doctrine forbids abortion(Boyle 1). It teaches that only God gives and takes life. Christianity also teaches that human life starts at conception, not birth. By effecting the abortion, Elsie will have effectively destroyed a human life.  

Methods of DNA Identification

Methods of DNA Identification To isolate DNA from blood, saliva, buccal swab and betel quid by phenol-chloroform method and chelex method and compare the efficacy of both the methods. To carry out restriction digestion of the DNA samples isolated from above mentioned sources using the restriction enzyme EcoRI (G|AATTC) and identify individuals based on the pattern of restriction banding and to ascertain the applicability of the restriction digestion in forensics MATERIALS AND METHODS: Blood, saliva, betel quid and buccal swab were collected from 15 patients and DNA isolation was done by phenol-chloroform method and chelexmethod. DNA fingerprinting was carried out using EcoRI restriction enzyme. RESULTS:  DNA could be extracted from residues of saliva, DNA fingerprinting done with the isolated DNA was able to match with those of individuals. Chelex method was found to be more efficient than the Phenol-chloroform method KEY WORDS: Betel Quid, Chelex method, DNA,DNA fingerprinting,Phenol chloroform method Introduction DNA fingerprinting  has ascertained an increasingly imperative role towards decision making in judiciary. DNA tests have helped convict suspects, to exonerate suspects or overturned previous convictions. Scientific evidences such as fingerprints, blood, semen, shreds of clothing, hair, weapons, tire tracks, and other physical evidence at the crime scene can be a more riveting to a tribunal than the testimony of an eyewitness. DNA is more suitable because DNA remains scathe lessin challenging environments where such evidence is found. The DNA molecule holds an impressive dependability to withstand time. 1 DNA profiling compares the DNA fragment lengths and patterns. The isolated DNA from the samples is fragmented using a restriction enzyme. Then the length of the resulting fragments is determined by electrophoresis and comparedby a visual interpretation of the pattern of DNA bands. 2 DNA can be sourced from freshblood, fresh or dried human buccalswabs, soft tissue, saliva and salivary stains. Optimizing the methodology in DNA extraction from various sources have been tried by many studies. Minute quantities of saliva allows establishing DNAprofile. 3DNA has been proven to be isolated from cell samples from objects that was in contact with the body and from sources like chewing gums, cigarettes, bite marks in foods, among others. Restriction fragment length polymorphism (RFLP) analysis provides details of the DNA which is referred as a DNA fingerprint. As DNA is unique to every individual, analyzing the sequence helps in identification of specific patterns of each individual. DNA profile is considered as valid evidence in the court of law for paternity disputes and human identification. Standardization of DNA extraction technique will improve the reliability and speed up sample processing time. 4-6 Limited availability of biological samples in a crime scenechallenges the procedure of extraction , characterization and analysis of DNA. Furthermore, difficulty arises in retrieving DNA from stains and degraded samples which provide contaminated or poor qualityDNA. Hence, purification of DNA from samples is still a significant step in obtaining useful genotypes. Notwithstanding, tremendous advances have been made in the recent times in DNA testing. 7 Chewed betel quid (BQ) stains are encountered frequently on crime scenes in Southeast Asian countries. Though the quid presents as an important biological evidence, the forensic analysis using betel quid as an evidence has been impeded due to difficulty in extraction of human DNA . Hence, constituting a definite method for extracting DNA from chewed Betel quid residues is of paramount importance. 8 Saliva found on victims of several violent crimes is a potential source of DNA. They can be recovered from bite marks, cigarette butts, betel quid, postage stamps, envelopes and other objects. However , salivary stains usually dry up easily becoming invisible, making recognition and collection difficult. Among the various biological sources available, salivary analysis have great discriminatory power and can be incorporated into a criminal investigation . Improvisation of DNA extraction procedures will improve its reliability and also help to expedite the process. The present study aims to isolate DNA from blood, saliva (under different conditions) by phenol chloroform method and chelex method and to find the efficacy of these methods in extraction of DNA from traces of saliva. 9,10 ISOLATION OF DNAFROM BLOOD AND SALIVA BY PHENOL CHLOROFORM METHOD : The DNA was extracted with an equal volume of phenol: chloroform: isoamyl alcohol. This mixture was centrifuged at 10000rpm for 5 minutes. The aqueous phase was collected and extracted with chloroform: isoamyl alcohol mixture and centrifuged at 10000rpm for 5 minutes. The supernatant was transferred to a new microfuge tube and 0. 6 volume of isopropanol was added. The spongy white precipitate was transferred to a microfuge tube and added equal volume of ethanol was added. Then it was centrifuged at 10000rpm at room temperature for 10 minutes. The supernatant was drained and to the pellet 100 µL of TE buffer was added stored at 4 °C. ISOLATION OF DNAFROM BLOOD AND SALIVA BY CHELEX METHOD: 0. 5 ml of whole blood was collected in 2 ml tube and the cells are harvested by centrifugation at 3000 rpm for 3 min. at 4 °C. The supernatant was discarded. 0. 8 ml TBP buffer was added to the collection tube, vortexed gently, then centrifuged at 3000 rpm for 3 minutes, supernatant was discarded. The next stepwas continued if the blood pellet looks mauve 0. 5 ml of TBM buffer was added to the tube, and vortexed followed by addition of 3  µLof proteinase K and incubated at 55 °C for 30 minutes. The sample was centrifuged for 2 minutes at 5000 rpm and the supernatant saved to 2 ml tube and then added 260  µL of absolute ethanol. The mixture was applied to EZ-10 column, centrifuged at 8000 rpm for 1 minute; discarded the flow in the collection tube. 500  µL of wash solution was added and centrifuged at 8000 rpm for 1 minute. This step was repeated spin at 8000 rpm for an additional minute to remove residual amount of wash solution. The column was placed into a clean 1. 5 ml microfuge tube and 30  µL of elution buffer was added into the center part of membrane . The tube was incubated at 50 °C for 2 minutes centrifuged at 10,000 rpm for 1 minute to elute the DNA from the column The standards and samples were removed from the freezer and thawed. In a separate sterile 1. 5 ml microfuge tube for each standard/sample, 10  µl of DNA was mixed with 990  µl of D. I. water and vortexed . The solution was allowed to stand for 10 minutes to ensure the complete diffusion of DNA throughout the solution. This represents a 1:100 dilution of the standards and the DNA samples. B. DNAquantification The DNA sample was briefly vortexed and the solution wastransfered to the cuvette of the spectrophotometer with care not to create bubbles. The cuvette is inserted into the spec ensuring the correct face of the cuvette is in line the light beam. . An absorbance reading appears on the screen . Reading is continued until all standards and samples have been quantified. The concentration of DNA in the sample is determined according to the conversion factor (A260 of 1. 0 = 50  µg ml-1 DNA). The concentration of DNA in the sample can be read as  µg/mL using the conversion factor and dilution factor . RESTRICTION DIGESTION: Restriction enzyme buffer was vortexed before pipetting to ensure that it was well-mixed and was added to the tube . Appropriate amount of DNA to be cut wasvortexed before pipetting to ensure that it was well-mixed and was added to the tube. After vortexingthe enzyme to ensure that it was well-mixed 1 ÃŽ ¼L of enzyme EcoRIwas added. The mixture is placed in thermal cycler (Eppendorf) for2-3 hour incubation at 37 °C . To heat inactivate the enzyme the mixture is maintained at 80 °C for 20 min. The mixture is kept at 4 °C until the reaction mixture is out of the thermal cycler. Agarose Gel Electrophoresis Protocol Preparation of the agarose gel 1. 25 g Agarose powder was taken in 500 ml flask and 125 ml of TAE Buffer was added to it. The mixture is melted in hot water bath till a clear solution forms. The solution is allowed to cool to a temperature of 50-55 °C by periodic swirling to achieve even cooling. To it ethidium bromide solution was added. The ends of the casting tray are sealed with two layers of tape. The combs are placed in the gel casting tray. The melted agarose solution was poured into the casting tray and allowed to cool until it is solid. The comb and the tape are removed carefully. The gel is placed in the electrophoresis chamber. 2-3 mm of TAEBuffer is added over the gel. Loading the gel 6 à ¯Ã‚ Ã‚ ­l of 6X Sample Loading Buffer is added to each DNA sample containing tubes. 20 à ¯Ã‚ Ã‚ ­l of each sample is pipetted into separate wells in the gel. 10 à ¯Ã‚ Ã‚ ­l of the DNA ladder standard is pippeted into one well of each row on the gel. Running the gel The lid is place on the gel box, the electrode wires are connected to the power supply. The power supply is turned on to about 100 volts. To ensure the correct direction of the current, the movement of the blue loading dye is checked. The power supply is continued till the blue dye approaches the end of the gel. The wires are disconnected from the power supply. The lid is removed from the electrophoresis chamber. Using gloves, gel is carefully removed and observed in a transilluminator for the DNA bands. RESULTS: Isolation of DNA was done from blood ,fresh saliva, saliva stored at -20 °C, saliva stored at 37 °C for 24hrs ,buccal swab and betel quid by both the phenol-chloroform method and the chelex method. . Gel electrophoresis of the isolated genomic DNA was carried out on 0. 8% agarose gel. (figure 1) After restriction digestion electrophoresis gel is prepared to run and to identify the number of bands. DNA samplesobtained from blood were labelled as Aband subsequently as Bb,Cb, DbEbas shown in table 2. DNA obtained from fresh saliva were labelled as As,Bs, Cs, Ds,Es. DNA obtained from saliva stored at -20 degree were labeled as AfsBfsCfsDfs. Efs. DNA obtained from saliva stored at room temperature were labelled as Ads,BdsCdsDdsEds DNA obtained from bloodof 5 individuals was made to run in the well marked 1 to 5 in a uniform manner ie DNA obtained from the first individual named as Ab, was made to run in well No. 1 . DNA obtained from second individual named as Bb was made to run in well No. 2 DNA obtained from third individual named as Cb was made to run in well No . 3. DNA obtained from fourth individual named as Db was made to run in as well No- 4. DNA obtained from Fifth individual namedEbwas made to run in well No. 5. (table 2) But while running DNA obtained from saliva of different sources the order was changed randomly. For example DNA isolated from fresh saliva for the first individual (As) instead of being run in the first well ie well No -6 was made to run in the third well( well no 8) and DNA isolated from saliva stored at -20 degree for the first individual(Afs) instead of being run in the first well ie well No-11was made to run in the third well (well No. 13)and DNA isolated from saliva stored at room temperature for the first individual(Ads) instead of being run in the first well ie well No-16 was made to run in the fifth well (well No. 20). Likewise DNA isolated from different sources of saliva of different individuals made to run in different wells and the number of bands produced is identified . From the figure 1 it could be identified that the well number 1,8 ,13,20 corresponding to DNA isolated from the first individual from various sources named Ab AsAfs Ads identified by the yellow arrow has uniformly three bands. For the well number 2,7,14,19 corresponding to DNA isolated from the second individual from various sources named BbBsBfsBds identified by the blue arrow has uniformly 6 bands . various DNA isolated from the fifth individual from various sources namedEbEsEfsEds identified by the green arrow has uniformly 4 bands . From the above figure itcould be identified that the well number 1,10 corresponding to DNA isolated from different source for the first individual named Ab,AbS,identified by the yellow arrow has uniformly four bands. For the well number 2 and 6 corresponding to DNA isolated from second individual from blood and buccal swab named BbBbSl identified by the blue arrow has uniformly 6 bands . For the well number 3 and 7corresponding to DNA isolated from third individual from blood and buccal swab namedCbCbS identified by the red arrow has uniformly 5 bands . For the well number 4and 8 corresponding to DNA isolated from fourth individual from blood and buccal swab named DbDbs,identified by the aqua arrow has uniformly 7 bands. For the well number 5and 9 corresponding to DNA isolated from fifth individual from blood and buccal swab named EbEbsE identified by the green arrow has uniformly 8 bands . This shows that DNA obtained from an individual from blood and buccal swab produce uniform banding pattern. This shows that DNA obtained from an individual from various source produce uniform banding pattern . Identification of individual from traces of saliva which could be used for forensic application -Extraction of DNA from Buccal swab. Restriction digestion with Ecor-1 from extracted DNA obtained from above mentioned source has been done for identifying individuals. Blood was used as a control and compared with DNA bands from buccal swab. A total of 10 wells were created. DNA obtained from blood wer e labeled as Ab, Bb,Cb, Db,Ebas shown in tab 3. DNA obtained from Buccal swab were labeled as Abs,Bbs, Cbs, Dbs, Ebs. DNA obtained from blood from 5 individuals was made to run in the well marked 1 to 5 in a uniform manner. But while running DNA obtained from buccal swab the order was changed randomly. For example DNA isolated from buccal swab for the first individual (Abs) instead of being run in the first well ie well No -6 was made to run in the fifth well( well no 10). Likewise DNA isolated from buccal swab of different individuals was made to run in different wells and the number of bands produced is identified Different methods of DNA extraction is been followed in that, most widely used is phenol chloroform method . Many new methods of DNA extraction have been tried. The chelex method is one among then . To know the efficacy of the chelex method it was compared with that of phenol chloroform method. Of the two methods studied the chelex method proved to be more easy to handle and less time consuming in addition to yieds higher amount of DNA and is proved by quantification with U. V spectrometer as shown in fig. 2. DISCUSSION: Forensic odontology is a branch of forensics which analyses stains and organic liquids from the oral cavity or its contents, bite mark comparison, investigation of trauma and oral injuries such as personal injury cases, and dental malpractice. The fundamental requirement of a criminal investigation is that the victim and aggressor should be positively identified. Forensic dentistry aids in the forensic process by comparing the deceaseds dentition with that of previous dental records or by facilitating to shape the profile of an individual in terms of age at the time of death, sex and phylogeny to aid in identification. 11,12 Saliva has been a potential source of identification and is usually found in bite marks, cigarette butts, betel quid, postage stamps, envelopes and other objects. The first phase of the study intended to isolate DNA from saliva (under different conditions), by phenol-chloroform method and chelex method and compare the yield with that of blood . The second objective was to find out efficacy of these methods in extraction of DNA from traces of saliva ie from Buccal swab, and from Betel quid and which could be used for forensic application. 8 The presence of residues are considerably important as biological evidences, but forensic analysis of such evidences has been hindered by failures in extraction of human DNA. Consequently, it is indispensable in forensic science to establish a reliable method for extracting DNA from samples collected at the crime site. The most important objective was whether individuals can be identifed from samples of different source and to ascertain the applicability of the restriction digestion in forensics. 13,14 Blood was taken as control, saliva was divided into 3 parameters ie from fresh saliva, from saliva stored at -20 degree for24 hr from saliva stored in room temperature for 24 hr’s were obtained . Identification of individual has been done with restriction enzyme EcoRI. . The isolated DNA was digested using the restriction enzyme EcoRI(G|AATTC)The digested DNA was run on 1% agarose gel electrophoresis and the bands produced in each individuals DNA were scored and is proved that identification of individual can also be done by DNA fingerprinting or profiling. Agarose gel electrophoresis separates DNA fragments according to their size. The most important objective was whether individuals can be identified from samples of different source and to ascertain the applicability of the restriction digestion in forensics. 16 DNA fingerprinting is a technique that is used to represent like and unlike DNA that is present in different individuals. Nucleotide sequences which show significant variation from one individual to another are taken into consideration. 17The most important objective of the study was to ascertain whether individuals can be identified from samples of different source and to ascertain the applicability of the restriction digestion in forensics and the last objective was toCompare the DNA yield from manual and kit method. To prove that DNA could be extracted from traces of saliva , Buccal swab and Beetal quid was used . DNA could be extracted from buccal swab,beetal quid and quantification was done with U. V spectrometer. Comparison of DNA isolated from all the samples collected from all the individual using two different procedures has been done and comparison of yield of different sources showed the kit method to be more effective . Use of biological evidences like saliva, buccal swab and betel quid are compromised due to the quandary in extraction of human DNA. The present study had proved to establish a reliable method for extracting DNA from samples collected from different sources of saliva and from traces of salivary stains which was comparable to bloodin proving identification. Samples collected from different sources of saliva and from traces of salivary stains can also be assessed by DNA fingerprinting or profiling which is based on the fact that DNA is unique to every individual .

The Effects of Divorce on Children Essay -- Divorce and Adolescent Dep

Introduction In America, about one in every two marriages will end in divorce. Around 60% of those divorcing couples have children. (Cherlin, 2012). Half of the marriages in America end in divorce, and more than half of those couples have children, which means that about every other divorce that is filed in America, a child is impacted. Between 850,000 and 950,000 divorces occur each year. (National Center for Health Statistics, CDC., 2014). Given that roughly 60% of those divorcing couples have at least one child, at least 510,000 children are affected a year. Estimates have been done to suggest that in the near future, 70% of divorces could involve children under the age of eighteen. (Block, Block, and Gjerde, 1986). Because of the large number of children in America having broken families, it is important to understand the effects of divorce on children’s' day to day lives so that they may be provided for in a proper and beneficial way. Changes in the Family There are many different outcomes that the effect of a divorce may have on a child. Though divorce isn't always a positive thing, sometimes there are scenarios where a family is better off this way. According to research, the bond maintained between parent and child is the main change that plays a factor on the child's outcome when a divorce happens. The relationships between parents and their children were found to be more influential than the parents’ marital status. Negative effects were null if relationships remained intact after the divorce. However, sometimes the ability to keep these relationships closely knit just isn't as simple as it was before the divorce. Keeping a relationship intact is especially difficult for the non-custodial parent. (He... ...du/wp-content/uploads/sites/28/2012/02/Cherlin_JMFmarriagepaper.pdf Harvey, J. H., & Fine, M. A. (2004). Children of divorce stories of loss and growth. Jeynes, W. (2002). Divorce, family structure, and the academic success of children. New York: Haworth Press. Jost, K., & Robinson, M. Children and divorce. CQ Researcher, 1, 349-368. Stuart, I. R., & Abt, L. E.,. (1972). Children of separation and divorce. New York: Grossman Frank Trovato, (1987). A Longitudinal Analysis of Divorce and Suicide in Canada. Journal of Marriage and Family., Vol. 49., No. 1, pp.193-203. Robert H. Aseltine, Jr. (1996). Pathways Linking Parental Divorce With Adolescent Depression. Journal of Health and Social Behavior., Vol. 37, No.2., pp.133-148. Satoshi Tsujimoto, (2008). The Prefrontal Cortex: Functional Neural Development During Early Childhood, Neuroscientist 14:345.